Towards Integrated Multi-sensor Array Chip for Bioprocess Monitoring

Promovendus/a: Nurul Izni Binti Rusli

Promotor(en): Prof. dr. Michael Kraft, Prof. dr. Irene Taurino

This study aims to enhance the monitoring of bioprocesses, specifically yeast fermentation, to improve efficiency and product quality. The researchers developed a microfabricated multi-sensor array chip capable of measuring multiple important parameters. A notable feature of the chip is the integration of platinum nanostructures on microelectrodes, enabling highly sensitive and selective measurements with minimal errors. The chip incorporates sensors for pH, conductivity (which indirectly assesses microbial growth and activity), dissolved oxygen, glucose levels, and temperature. It also includes built-in reference electrodes, eliminating the need for external ones. The chip's sensors demonstrated excellent performance in various tests, accurately measuring temperature and glucose levels in yeast fermentation samples. The use of platinum nanostructures improved sensor sensitivity, linearity, and resistance to interference. The chip's compact size allows for autonomous usage, making it suitable for real-time monitoring of bioprocesses. Future work will focus on further enhancing the chip's capabilities, such as incorporating anti-fouling coatings and integrated electronics for continuous fermentation process monitoring. Overall, this research represents a significant advancement in monitoring yeast fermentation and controlling bioprocess parameters, with potential applications in diverse fields.